LBC & HPV Detection and Typing by PCR Test
LBC & HPV Detection and Typing by PCR Test
Thin-layer liquid-based cytology(LBC) and HPV Detection and Typing by PCR is a co-test to examine cervical cell abnormalities and identify and differentiate high and low-risk strains of Human papillomavirus (HPV) in cytological samples.
The process usually begins with using LBC, an improved screening technique that involves collecting cervical tissue samples in a liquid vial for accurate analysis, potentially preventing unnecessary preventive treatment. This is followed by HPV testing, which is conducted using the polymerase chain reaction (PCR) technique to detect and type high-risk HPV DNA( Type 16 and Type 18) in the cells of the cervix.
The approach helps handle three clinical conditions:
- Screen women of all age groups for cervical cancer and access their HPV infection status
- Manage women with mild or borderline smear results
Monitoring treatment for those already diagnosed with precancerous or neoplastic lesions.
What is the LBC and HPV Detection and Typing by PCR test Used For?
A doctor might request a LBC and HPV Detection and Typing by PCR test
To provide comprehensive screening of cervical cancer in women over 30 years of age
To confirm abnormal or inconclusive Pap smear results
To guide treatment choices and plan follow up care in patients diagnosed with HPV infection
To monitor treatment response in women with precancerous and neoplastic lesions
Symptoms of Cervical Cancer
For early detection, diagnosis, and treatment of cervical cancer, women must keep an eye on these symptoms
Foul-smelling vaginal discharge
Unusual Bleeding from the vagina after menopause or sexual intercourse
Itching in the affected area
Constant fatigue
Changes in the skin color around the genitals
Weight loss
Persistent pain in the back, legs, or pelvis
Preparation for Test
No specific preparation is required for LBC plus HPV detection and typing by PCR. However, you may be asked to refrain from sexual activity or the use of vaginal medications or tampons for 48 hours before taking the test, as they can influence the accuracy of the test results.
Interpretation of Test Results
The report, which helps you to comprehend LBC plus HPV detection and typing by PCR results, is interpreted as follows:
Positive (abnormal) results
This report indicates precancerous or cancerous changes in the cervical cells with the presence of high-risk HPV types. Your doctor may request a cervical biopsy for further analysis and accurate interpretation.
Negative (Normal) results
This report suggests no unexpected cervical changes in the tissue sample, although the test must be repeated every 3- 5 year for women over 30 years.
FAQs
When is an LBC + HPV DNA Detection and typing by PCR test performed?
This test is recommended in the following scenario:
Screening for the virus in women aged between 30 and 65 years who have no symptoms and never had cervical screening before.
Pap smear results show abnormal cells in the cervix.
People with previous low-grade results who find follow-up difficult and have not returned to regular screening
Is LBC better than a Pap smear test?
Unlike the conventional Pap smear, LBC involves collecting the cervical sample in a liquid medium that directly improves the cervical cancer diagnosis. The examination is also more accurate and less invasive. It is also less likely to produce false positive results when screening women for cervical cancer.
How does HPV infection spread?
The HPV infection usually spreads during vaginal or anal sex from someone who has the virus. It can also spread through skin-to-skin contact during sex.
How long does it take to get the results?
The report may take about 10 -14 days after the test. If the results indicate something abnormal, your doctor will contact you to discuss the next step.
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