HPV HR DNA Detection by Real-Time PCR
HPV HR DNA Detection by Real-Time PCR
HPV (Human Papillomavirus) infection is a viral infection that frequently results in the production of warts on the skin or mucous membranes. There are around 100 HPV genotypes that can cause an impact on an individual, with a few causing warts and a few high-risk genotypes causing cervical cancer. It primarily affects women. Males can contract the disease, but they are more likely to transmit it to their female sexual partners during intercourse.
The HPV HR (HPV high-risk) DNA detection by real-time PCR test involves measuring gene expressions of various strains of HPV, which include 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, and 68, that have been linked to cervical cancer incidence. Among all these, the HPV 16 and 18 genotypes are the most closely linked to the advancement of cervical cancers in women. This test can help identify high-risk genes linked to the development of cervical cancer in women
Symptoms of HPV infection
Most people don’t even realize or have any symptoms due to HPV infection. However, a few symptoms include genital warts and lumps around the vagina, anus, or penis. The common symptoms of HIV include
Fever
Chills
Rash
Night sweats
Muscle aches
Sore throat
Fatigue
Swollen lymph nodes
Mouth ulcers
Who should get tested?
The HPV HR DNA detection test is recommended for women who are/have:
Age 30 years or above
HPV positive
History of abnormal pap smear
Other STDs such as HIV
Pregnant and were exposed to diethylstilbestrol (DES)
Weakened immune system
Test preparation
No special preparations are required before taking the HPV HR DNA detection test.
Please inform your healthcare provider if you have/are on any medications, supplements, or any other medical history before taking the test.
Avoid intercourse, vaginal spreads, spermicidal foams, douches, etc., 2 days before taking the test.
Specimens should not be collected during, before, or shortly after the menstrual period.
Interpretation of the Test Results
The HPV HR DNA detection test results are usually given as either positive or negative.
Positive test results – These results show the presence of HPV due to 1 or more genotypes in the samples, indicating an increased risk of getting cervical malignancies sooner. Individuals who get positive test results will require colposcopy.
Negative test results – These indicate the absence of different types of HPV genotypes. Individuals who are at risk of HPV but with negative test results are referred for repeat testing by both cytology and an HR-HPV test within 12 months
FAQs
What is the turnaround time (TAT) for the HPV HR DNA detection test?
The HPV HR DNA detection test results are generally available within 4 – 6 working days after the sample collection. However, it could take a little longer at times
What is the test procedure involved in sample collection for the HPV HR DNA detection test?
The test procedure involves collecting samples by gently inserting a speculum (tiny device) into the vagina to clearly view the cervix. To collect the sample, a small brush, spatula, or scraper is used. It is typically a painless process and takes about a few seconds in sample collection
Are there any risks associated with the HPV HR DNA detection test?
No, there are no potential risks associated with the HPV HR DNA detection test. The procedure is painless and quick and simple to conduct. However, you may experience spotting or some discharge for a short time after the sample collection, which is normal
What are the risk factors for HPV infection?
A few risk factors for HPV infection include:
· Multiple sexual partners
· Sexually more active
· Weakened immune system
· Close contact with infected individuals
· Other STDs (HIV, Hepatitis-B, etc.)
How can one avoid contracting HPV or genital warts?
HPV vaccination can help prevent nearly all HPV infections, including genital warts. Furthermore, using condoms during sexual intercourse helps protect against HPV infection. However, condoms cannot provide 100% protection against the virus since they do not cover the entire skin
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