Clostridium Difficile Detection PCR
Clostridium Difficile Detection PCR
The Clostridium difficile detection PCR test is a molecular method used to identify the presence of the bacterium, Clostridium difficile in stool samples. It employs polymerase chain reaction (PCR) technology to amplify specific regions of the bacterium's DNA, allowing highly sensitive and specific detection. This test is crucial for diagnosing C. difficile infections, particularly in healthcare settings where rapid and accurate identification is essential for effective patient management and infection control measures.
Thus, getting this test done is recommended to diagnose the Clostridium difficile infection
Symptoms of C.difficile infection
Watery diarrhoea three or more times a day with unknown cause
Bloody diarrhoea
Abdominal pain
Nausea and vomiting
Loss of appetite
Fever
Who should get tested?
C. difficile detection PCR test is recommended for individuals who are at increased risk of developing the infection. Following are the various risk factors that make an individual susceptible:
Age over 65 years
Has symptoms associated with C. difficile infection
Has taken antibiotics within the last 6-8 weeks
Has recently stayed in or worked at a hospital or any health care facility (C. difficile bacteria is more common in healthcare facilities)
Has a weakened immune system from a health condition such as HIV or certain medicines or treatments, including chemotherapy for cancer
Test preparation
No special preparation is required for the C. difficile detection PCR test.
Interpretation of the Test Results
The test results may vary depending on the age, gender, medical history, and procedure. C. difficile detection PCR test results may be positive or negative.
Positive
Positive results for C. difficile detection PCR test indicate the presence of toxins A & B produced by the bacteria in the stool sample. This suggests an active C. difficile infection.
Negative
A negative result indicates the absence of toxins A & B produced by the bacteria in the stool sample
FAQs
How is the sample collected for the C. difficile detection PCR test?
For the C. difficile detection PCR test, a stool sample is collected using a clean container. The patient is instructed to defecate directly into the container or to transfer a small portion of stool into it using a provided scoop
What is the turnaround time (TAT) for a C. difficile detection PCR test?
The test results for C. difficile detection PCR test are usually available within the same day as the sample collection. However, this duration may vary depending on the diagnostic clinic
Are there any risks associated with the C. difficile detection PCR test?
There are no risks associated with C. difficile detection PCR test. You are just instructed to collect your regular stool sample for testing which involves no risk
What might affect my C. difficile detection PCR test results?
Contamination of the stool sample with toilet water, urine, or other substances can make it unsuitable for testing or potentially alter the results. Thus, it is important to collect the sample without any such contaminants to ensure the accuracy of the test results
How can I prevent C. difficile infection?
To prevent C. difficile infection, practice good hand hygiene by washing hands thoroughly with soap and water. Avoid unnecessary antibiotic use, as it disrupts the gut microbiota. Clean surfaces regularly with bleach-based disinfectants. Use contact precautions in healthcare settings. Ensure proper sanitation and hygiene in food preparation. Finally, encourage vaccination, if available. These measures collectively reduce the risk of C. difficile transmission and infection
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